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A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
JoVE Journal
Chemistry
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JoVE Journal Chemistry
A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

DOI:
10.3791/50689-v

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10:44 min

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August 26, 2013

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,
1Department of Chemistry and Chemical Biology,McMaster University

Chapters

  • 00:05Title
  • 01:44Forming a Microarray Using Printable Protein-doped Sol-gels
  • 05:22Acetylcholinesterase Activity Assay
  • 06:52Microarray Imaging and Analysis
  • 08:13Results: Quantitative Sol-gel Derived Microarrays to Measure Inhibition
  • 10:16Conclusion

Summary

Automatic Translation

Automatic Translation

A guided material screening approach to develop sol-gel derived protein doped microarrays using an emerging pin-printing method of fabrication is described. This methodology is demonstrated through the development of acetylcholinesterase and multikinase microarrays, which are used for cost-effective small-molecule screening.

Tags

Materials ScreeningSol-gelProtein MicroarrayEnzyme AssayAcetylcholinesteraseKinasesQuantitative ScreeningDose-response CurvesHigh-throughput

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