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Isolation, Culture, and Transplantation of Muscle Satellite Cells
JoVE Journal
Biology
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JoVE Journal Biology
Isolation, Culture, and Transplantation of Muscle Satellite Cells
DOI:

10:25 min

April 08, 2014

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Chapters

  • 00:05Title
  • 02:07Isolation of Mononuclear Cells from Mouse Skeletal Muscle
  • 04:19Antibody Staining and Separation with MACS
  • 06:39Myoblast Differentiation and Transplantation for Skeletal Muscle Regeneration
  • 08:50Results: Representative Muscle Satellite Cell Transplantation
  • 09:52Conclusion

Summary

Automatic Translation

The isolation and culture of a pure population of quiescent satellite cells, a muscle stem cell population, is essential to the understanding of muscle stem cell biology and regeneration, as well as stem cell transplantation for therapies in muscular dystrophy and other degenerative diseases. 

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