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Journal / Bioengineering / Utilizzando Chips microfluidica per l'imaging …
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Utilizzando Chips microfluidica per l'imaging dal vivo e studio delle risposte lesioni in
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Utilizzando Chips microfluidica per l'imaging dal vivo e studio delle risposte lesioni in

Article DOI: 10.3791/50998-v • 11:46 min • February 7th, 2014
February 7th, 2014 •
1, 2, 1, 1, 1, 3,4, 2,5, 1
1Department of Molecular, Cellular and Developmental Biology, University of Michigan, 2Department of Biomedical Engineering, University of Michigan, 3Life Sciences Institute, University of Michigan, 4Department of Cell and Developmental Biology, University of Michigan, 5Department of Mechanical Engineering, University of Michigan

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Drosophila larve sono un sistema modello attraente per l'imaging dal vivo a causa della loro cuticola trasparente e potenti genetica. Questo protocollo descrive come utilizzare un dispositivo PDMS singolo strato, chiamato il 'chip di larva' per l'imaging dal vivo di processi cellulari all'interno dei neuroni di 3 ° instar larve di Drosophila.

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Bioingegneria , Live Imaging microfluidica danno assonale la degenerazione assonale imaging calcio fotoconversione microchirurgia laser
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