Journal
/
Bioengineering
/
Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

DOI:
10.3791/51215-v

•

10:13 min

•

June 03, 2014

•

, , , , ,
1The BioMEMS Resource Center, Department of Surgery,Massachusetts General Hospital, 2Harvard Medical School, 3Shriners Burns Hospital, 4Harvard University School of Engineering and Applied Sciences

Chapters

  • 00:05Title
  • 02:22Microfluidic Device Fabrication
  • 04:19Microfluidic Assay Preparation
  • 06:11Sample Preparation: Human Neutrophils From Capillary Blood
  • 06:49Microscopy and Image Analysis for Neutrophil Chemotaxis Measurements
  • 07:53Results: Neutrophils Chemotaxis Assay From Whole Blood
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol details an assay designed to measure human neutrophil chemotaxis from one droplet of whole blood with robust reproducibility. This approach circumvents the need for neutrophil separation and requires only a few minutes of assay preparation time. The microfluidic chip enables the repeated measure of neutrophil chemotaxis over time in infants or small mammals, where sample volume is limited.

Tags

Microfluidic PlatformNeutrophil ChemotaxisUnprocessed Whole BloodNeutrophil MigrationBlood DropletChemoattractantRed Blood Cell FiltrationFinger PrickVenous BloodPurified Neutrophils

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

High-throughput Protein Expression Generator Using a Microfluidic Platform

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

The Multi-organ Chip – A Microfluidic Platform for Long-term Multi-tissue Coculture

Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Read Article