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Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
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1Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research,Westmead Hospital, 2Centre for Research Excellence in Critical Infection, Westmead Millennium Institute,Westmead Hospital, 3Sydney Emerging Infectious Diseases Institute, University of Sydney,Westmead Hospital
Chapters
- 00:05Title
- 01:20Preparation of Bacterial Inoculate from Blood Culture Broth
- 05:46Preparation of MALDI-TOF Target Plate
- 06:34MALDI-TOF MS Spectra Acquisition
- 08:42Post Analysis-Interpretation of MALDI-TOF MS Scores
- 10:18Representative MALDI-TOF Analysis of Gram Negative Bacteria from Blood Cultures
- 12:35Conclusion
The application of matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth expedites the identification of bacteria. The presented method is a rapid and reliable method for identification of Gram negative bacteria directly from blood culture broth.
Tags
Rapid IdentificationGram Negative BacteriaBlood Culture BrothMALDI-TOF Mass SpectrometryClinical MicrobiologyBacterial IdentificationBloodstream InfectionSub-cultureSolid AgarMALDI-TOF MSBlood Culture SpecimensInterfering ResinsProteinsHemoglobinBacterial ConcentrationBacterial PurificationBacterial Extraction
