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Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
DOI:

08:09 min

April 06, 2015

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Chapters

  • 00:05Title
  • 02:11In Vivo Imaging
  • 03:07Harvesting and Preparation of Tumors
  • 04:19FACS Analysis
  • 06:01Results: Nanobody and mAb Staining of Tumors
  • 07:09Conclusion

Summary

Automatic Translation

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.

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