02:43Collection of Blood and Preparation of Blood Spots from Skin Puncture
04:38Drying and Storage of Dried Blood Spots
05:40Elution of Dried Blood Spots and Analysis of Eluates
07:39Results: Testing of Dried Blood Spot Eluates for Markers of Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), and Human Immunodeficiency Virus (HIV) Infections
The preparing and processing of dried blood spots (DBS) for their final analysis are still poorly standardized for most diagnostic applications. To overcome this shortcoming, a comprehensive step-by-step protocol is suggested and subsequently evaluated with regard to its effectiveness for detecting markers of viral infections.