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Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

DOI:
10.3791/53073-v

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09:38 min

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December 01, 2015

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, , , , ,
1School of Materials Science and Engineering,Nanyang Technological University, 2Centre for Biomimetic Sensor Science,Nanyang Technological University, 3School of Chemical and Biomedical Engineering,Nanyang Technological University

Chapters

  • 00:05Title
  • 01:22Formation of Supported Lipid Bilayer on a Hydrophilic Solid Support and Cholesterol-enriched Supported Membranes
  • 03:05Membrane Fluidity Assay
  • 05:01Cholesterol Quantification Assay
  • 07:51Results: Fabrication of Planar Bilayer on Solid Support Using SALB Method
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

We present an experimental protocol to form a supported lipid bilayer on solid substrates without using lipid vesicles. We demonstrate a one-step method to form a lipid bilayer on silicon dioxide and gold as well as supported membranes with cholesterol-enriched domain for various biological applications.

Tags

Biomembrane FabricationSolvent-assisted Lipid BilayerSALB MethodSupported Lipid BilayerSLBVesicle FusionLipid DepositionOrganic SolventHydrophilic SurfaceCholesterol-rich Membranes

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