Journal
/
/
Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
DOI:

09:38 min

December 01, 2015

, , , , ,

Chapters

  • 00:05Title
  • 01:22Formation of Supported Lipid Bilayer on a Hydrophilic Solid Support and Cholesterol-enriched Supported Membranes
  • 03:05Membrane Fluidity Assay
  • 05:01Cholesterol Quantification Assay
  • 07:51Results: Fabrication of Planar Bilayer on Solid Support Using SALB Method
  • 08:44Conclusion

Summary

Automatic Translation

We present an experimental protocol to form a supported lipid bilayer on solid substrates without using lipid vesicles. We demonstrate a one-step method to form a lipid bilayer on silicon dioxide and gold as well as supported membranes with cholesterol-enriched domain for various biological applications.

Related Videos

Read Article