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Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
JoVE Journal
Biology
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JoVE Journal Biology
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
DOI:

10:05 min

January 20, 2016

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Chapters

  • 00:05Title
  • 00:48Crosslinking in Live Cells
  • 03:19Isolation of Chromatin
  • 05:14Capture of Ligand-DNA Crosslinks and Isolation of Affinity-purified DNA
  • 07:52Results: COSMIC Indentifies Genome-wide Binding Events of Polyamides
  • 09:21Conclusion

Summary

Automatic Translation

Identifying the direct targets of genome-targeting molecules remains a major challenge. To understand how DNA-binding molecules engage the genome, we developed a method that relies on crosslinking of small molecules to isolate chromatin (COSMIC).

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