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Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
DOI:

06:35 min

January 10, 2016

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Chapters

  • 00:05Title
  • 00:42Extraction of Fibroblasts from Ear and Tail Tissues
  • 03:20Cell Culture and Sub-Culturing of Fibroblasts
  • 05:05Results: Fibroblast Culture Hardiness and Purity
  • 06:03Conclusion

Summary

Automatic Translation

We describe a simple and quick experimental procedure for generating primary fibroblasts from the ears and tails of mice. The procedure does not require special animal training and can be used for the generation of fibroblast cultures from ears stored at RT for up to 10 days.

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