Journal
/
/
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
DOI:

10:17 min

January 31, 2016

, , ,

Chapters

  • 00:05Title
  • 00:55Preparing the Embryos for Hybridization
  • 02:48Hybridization Probes to Prepared Embryos
  • 05:25Detecting the Probes and Staining
  • 07:59Results: Stain Precipitate Color Combinations
  • 09:42Conclusion

Summary

Automatic Translation

We describe a multi-target chromogenic whole-mount in situ hybridization (MC-WISH) procedure in intact Drosophila embryos allowing simultaneous and specific detection of three different mRNA distribution patterns by contrasting color precipitates.

Related Videos

Read Article