Journal
/
Developmental Biology
/
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

DOI:
10.3791/54016-v

•

09:31 min

•

June 10, 2016

•

, , , , , , , ,
1Diabetes and Metabolism Research Institute,Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences,Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering,California Institute of Technology

Chapters

  • 00:05Title
  • 01:22Adult Murine Pancreata Single-cell Suspension Generation
  • 04:33Colony Whole Mount Immunostaining
  • 07:39Results: Representative Analyses of Colonies Derived from PCFU (Pancreatic Colony Forming Unit)
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

In vitro colony assays to detect self-renewal and differentiation of progenitor cells isolated from adult murine pancreas are devised. In these assays, pancreatic progenitors give rise to cell colonies in 3-dimensional space in methylcellulose-containing semi-solid medium. Protocols for handling single cells and characterization of individual colonies are described.

Tags

In Vitro Colony AssayTri-potent Progenitor CellsAdult Murine PancreasPancreatic Progenitor CellsSelf-renewalDifferentiationHematopoietic Stem CellsPancreatic Cell PopulationsAbdominal CavitySpleenPBSBSADNase ICollagenase BCell IsolationCell Culture

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Read Article