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In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
DOI:

09:31 min

June 10, 2016

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Chapters

  • 00:05Title
  • 01:22Adult Murine Pancreata Single-cell Suspension Generation
  • 04:33Colony Whole Mount Immunostaining
  • 07:39Results: Representative Analyses of Colonies Derived from PCFU (Pancreatic Colony Forming Unit)
  • 08:39Conclusion

Summary

Automatic Translation

In vitro colony assays to detect self-renewal and differentiation of progenitor cells isolated from adult murine pancreas are devised. In these assays, pancreatic progenitors give rise to cell colonies in 3-dimensional space in methylcellulose-containing semi-solid medium. Protocols for handling single cells and characterization of individual colonies are described.

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