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Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
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Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

DOI:
10.3791/54063-v

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08:23 min

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June 23, 2016

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, , ,
1Department of Science and Mathematics,Alvernia University, 2711th Human Performance Wing, Air Force Research Laboratory,Wright-Patterson Air Force Base, 3UES, Inc.

Chapters

  • 00:05Title
  • 00:47Synthesis via Citrate Reduction of Gold Nanoparticles (AuNP) and Characterization
  • 02:35DNA-aptamer, Buffer, Solution, and Assay Preparation
  • 03:35Salt Titration and Assay Setup
  • 05:21Freezing Aptamer–AuNP Assay for Long-term Storage
  • 06:38Results: Assay Response with Changing DNA Coverage Density and Performance of the Assay for Long-term Storage
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

The design and development of an aptamer–gold nanoparticle colorimetric assay for the detection of small molecules for in-the-field applications was examined. Additionally, a smart-device colorimetric application (app) was validated and long-term storage of the assay was established for use in the field.

Tags

AptamerGold NanoparticleColorimetric AssayIn-field ApplicationReproducibilityShelf-lifeFalse PositiveGold ChlorideSodium CitrateDEPCAutoclaveCocaine-binding AptamerPhosphoramidite ChemistryOligonucleotide

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