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Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs)
JoVE Journal
Biology
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JoVE Journal Biology
Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs)
DOI:

07:42 min

June 20, 2016

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Chapters

  • 00:05Title
  • 00:34mRNA Transcription of Transcription Activator-like Effector Nucleases
  • 02:18Inject Astyanax mexicanus Embryos with TALEN mRNA
  • 04:06Phenotype Founder Fish and Evaluate TALEN Efficiency
  • 05:27Results: Germline Transmission of TALEN-induced oca2 Mutations in A. mexicanus
  • 06:47Conclusion

Summary

Automatic Translation

Gene-targeting mutagenesis is now possible in a wide range of organisms using genome editing techniques. Here, we demonstrate a protocol for targeted gene mutagenesis using transcription activator like effector nucleases (TALENs) in Astyanax mexicanus, a species of fish that includes surface fish and cavefish.

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