/
/
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:41High Capacity Magnetic Protein A or G Bead Antibody Capture
- 01:43Antibody Labeling Using Amine Chemistry
- 02:56Antibody Labeling Using Thiol Chemistry
- 04:46Results: Magnetic BEad Fluorescent Dye Labeling Results in a Highly Efficient Purified Antibody Recovery
- 05:39Conclusion
The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.
