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An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment
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An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment

An Acute Retinal Model for Evaluating Blood Retinal Barrier Breach and Potential Drugs for Treatment

DOI:
10.3791/54619-v

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09:33 min

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September 13, 2016

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, , ,
1Graduate School of Biomedical Sciences,Rowan University, 2Department of Cell Biology,Rowan University School of Osteopathic Medicine

Chapters

  • 00:05Title
  • 00:52Retinal Organotypic Culture
  • 02:31Preparing Samples for Immunostaining
  • 04:03Immunostaining
  • 05:01Image Analysis
  • 06:41Results: The Blood Retinal Barrier is Compromised upon Histamine Exposure and Rescued by LXA4 Treatment
  • 08:22Conclusion

Summary

Automatic Translation

Automatic Translation

A low-cost, easy-to-use and powerful system is established to evaluate potential treatments that could ameliorate blood retinal barrier breach induced by histamine. Blood vessel leakage, Müller cell activation and the continuity of neuronal processes are utilized to assess the damage response and its reversal with a potential drug, lipoxin A4.

Tags

Blood Retinal BarrierNeural Degenerative DiseasesEx Vivo Retinal ModelDrug ScreeningRetina DissectionHistamineLXA4PFASucroseFreezing Medium

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