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Immunology and Infection
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Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
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Immunology and Infection
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JoVE Journal Immunology and Infection
Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

DOI:
10.3791/55087-v

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10:39 min

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March 10, 2017

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, , , , , ,
1Division of Biomedical Science, Faculty of Medicine,University of Tsukuba, 2Department of Basic Biomedical Science,Universidad Europea de Madrid, 3Human Biology Program, School of Integrative and Global Majors,University of Tsukuba, 4Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa,Instituto de Salud Carlos III, 5Division of Microbiology, Department of Medicine, School of Medicine,Universidad Complutense, 6Biology of Gram-Positive Pathogens, Department of Microbiology,Institut Pasteur, Paris, France, 7ERL3526,CNRS, Paris, France

Chapters

  • 00:05Title
  • 00:51Conjugation Using the Filter-mating Method
  • 03:10Phage Amplification on the Donor
  • 04:43Measuring the Phage Titer
  • 06:01Phage Transduction
  • 07:54Transformation Assay
  • 09:41Results: Successful Horizontal Gene Transfer
  • 10:10Conclusion

Summary

Automatic Translation

Automatic Translation

We describe here three different protocols for the in vitro investigation of conjugation, transduction, and natural transformation in Staphylococcus aureus.

Tags

Horizontal Gene TransferStaphylococcus AureusConjugationTransductionNatural TransformationAntibiotic ResistanceCFR GeneDonorRecipientFilter MembraneSelective AgarColony PCRAntibiogramMICDisc Diffusion

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