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Chapters
- 00:05Title
- 00:44Ligand Conjugation to Magnetic Beads
- 02:58Application of Tensional Forces on the Ligand-coated Bead Bound Adhesion Receptors on the Dorsal Surface of Cells
- 05:49Results: Mechanical Tension Induces Adhesion Maturation
- 07:03Conclusion
Cell surface adhesions are central in mechanotransduction, as they transmit mechanical tension and initiate the signaling pathways involved in tissue homeostasis and development. Here, we present a protocol for dissecting the biochemical pathways that are activated in response to tension, using ligand-coated magnetic microbeads and force application to adhesion receptors.
