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Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

DOI:
10.3791/55536-v

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11:37 min

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July 03, 2017

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, ,
1Unité de Pathogénie Microbienne Moléculaire, INSERM U786,Institut Pasteur, 2Microbiologie et Maladies Infectieuses,Collège de France, 3Laboratory of Intestinal Immunity, Institut Imagine-INSERM UMR 1163,Université Paris Descartes-Sorbonne Paris Cité

Chapters

  • 00:05Title
  • 00:45Bacteria:Host Cell Challenge
  • 02:59Stress Granule Formation Indication and Immunofluorescent Analysis
  • 06:09Fluorescence Imaging Analysis
  • 08:47Results: Representative Stress Granule Analysis
  • 10:20Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for the qualitative and quantitative analysis of stress granule formation in mammalian cells after the cells are challenged with bacteria and a number of different stresses. This protocol can be applied to investigate the cellular stress granule response in a wide range of host-bacterial interactions.

Tags

ImmunofluorescenceStress GranuleBacterial ChallengeMammalian CellsCellular MicrobiologyPathogenStress Granule PathwayImage AnalysisS.flexneri M90TTryptic Soy AgarTryptic Soy BrothVirulence Gene ExpressionHeLa CellsInfection MediumGentamicin

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