Journal
/
/
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
DOI:

09:16 min

May 30, 2017

, , , ,

Chapters

  • 00:05Title
  • 01:06Preparation of 5 Liter and 1 Liter Fly Cages and Collection of Fly Embryos
  • 04:12Embryo Dechorionation
  • 05:15Preparation of Embryo Extract
  • 07:15Results: Preparation of Drosophila Embryo Extracts for Proteomics Experiments
  • 08:35Conclusion

Summary

Automatic Translation

The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).

Related Videos

Read Article