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Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation
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Chapters
- 00:05Title
- 00:57In Utero Electroporation
- 04:18Brain Dissection and Embedding for Organotypic Slice Culture
- 06:40Vibratome Sectioning and Slice Culture
- 08:47Ime-lapse Imaging
- 10:30Results: Migration Behavior of Bcl11a Mutant Upper-layer Cortical Neurons
- 12:40Conclusion
This protocol provides instructions for direct observation of radially migrating cortical neurons. In utero electroporation, organotypic slice culture, and time-lapse confocal imaging are combined to directly and dynamically study the effects of overexpression or downregulation of genes of interest in migrating neurons and to analyze their differentiation during development.
