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Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
DOI:

10:45 min

July 26, 2017

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Chapters

  • 00:05Title
  • 01:07Dissociation Protocol
  • 02:42Antibody Staining and Cell Sorting
  • 04:39Transfection
  • 05:57Ca2+ Imaging in Differentiated Cells and Image Analysis
  • 08:50Results: Myoblast Characterization and a SOCE Experiment
  • 09:54Conclusion

Summary

Automatic Translation

Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.

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