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Journal / Biochemistry / Bitki Protoplastlarından mRNA İnteraktom…
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Biochemistry

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JoVE Journal Biochemistry
Bitki Protoplastlarından mRNA İnteraktom Yakalama
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Bitki Protoplastlarından mRNA İnteraktom Yakalama

Article DOI: 10.3791/56011-v • 12:29 min • July 28th, 2017
July 28th, 2017 •
1, 1, 1, 1
1Department of Biology, KU Leuven

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Burada, Arabidopsis thaliana yaprak mezofil protoplastlarına uygulanan bir etkileşimli yakalama protokolünü sunuyoruz. Bu yöntem kritik olarak in vivo UV çapraz bağlanmaya dayanır ve fizyolojik bir ortamdan bitki mRNA'ya bağlanan proteinlerin izole edilmesine ve tanımlanmasına izin verir.

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Plant Biology yaprak mezofil protoplastları 254 nm UV kaynağı, haberci RNA-bağlayıcı proteinler oligo (dT) boncuklar haberci ribonükleoprotein kompleks açılımı SDS-PAGE gümüş boyama nano-LC-MS mRNA'ya bağlı proteom
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