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Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
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Neuroscience
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JoVE Journal Neuroscience
Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

DOI:
10.3791/56537-v

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08:08 min

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February 19, 2018

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, , ,
1Department of Chemistry and Chemical Engineering,Chalmers University of Technology, 2Department of Chemistry and Molecular Biology,University of Gothenburg

Chapters

  • 00:04Title
  • 00:50Single Cell Exocytosis Amperometry Experiments
  • 04:00Intracellular Electrochemical Cytometry
  • 06:47Results: The Effect of Osmotic Pressure on Exocytosis Activity
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

Osmotic stress affects exocytosis and the amount of neurotransmitter released during this process. We demonstrate how combining electrochemical methods together with transmission electron microscopy can be used to study the effect of extracellular osmotic pressure on exocytosis activity, vesicle quantal size, and the amount of neurotransmitter released during exocytosis.

Tags

– Osmotic Stress- Secretory Vesicles- Exocytosis- Amperometric Recording- Carbon Fiber Microelectrode- Cyclic Voltammetry- Chromaffin Cells- Patch Clamp- Faraday Cage- Microscope Heating Stage

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