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Cancer Research
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Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

DOI:
10.3791/57215-v

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05:22 min

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April 13, 2018

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, ,
1Koch Institute for Integrative Cancer Research at MIT, Department of Biology,Massachusetts Institute of Technology

Chapters

  • 00:04Title
  • 00:32Synchronization of RPE-1 hTERT Cells
  • 01:34Generation of Aneuploid Cells by Interference with the Activity of the Mitotic Kinase Mps1 and Removal of Aneuploid Cycling Cells to Enrich the ArCK Population
  • 03:36Results: In Vitro Isolation and Characterization of Aneuploid Cells with Complex Karyotypes (ArCK)
  • 04:35Conclusion

Summary

Automatic Translation

Automatic Translation

Aneuploidy leads to genome instability, which eventually produces cell cycle-arrested cells with complex karyotypes. This paper provides a simple and convenient method to isolate aneuploid cells with complex karyotypes that cease to divide.

Tags

Cell Cycle ArrestComplex KaryotypesAneuploid CellsCell SynchronizationRPE-1 HTERT CellsThymidineMps1 Inhibitor ReversineNocodazoleMitotic Shake-offArCK Cells

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