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Custom Engineered Tissue Culture Molds from Laser-etched Masters
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Custom Engineered Tissue Culture Molds from Laser-etched Masters
DOI:

08:56 min

May 21, 2018

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Chapters

  • 00:05Title
  • 01:02Vector Format Master Mold Formation
  • 02:28Laser Cut the Acrylic Master Molds
  • 03:47Preparing the PDMS Mold for Cell or Tissue Culture
  • 05:30Casting Collagen and Fibrin Hydrogel Tissues
  • 07:25Results: Mold Enhanced Alignment of Cardiac Tissue Constructs
  • 08:22Conclusion

Summary

Automatic Translation

Herein we present a rapid, facile, and low-cost method for fabricating custom polydimethylsiloxane molds that can be used for producing hydrogel-based engineered tissues with complex geometries. We additionally describe results from mechanical and histological assessments conducted on engineered cardiac tissues produced using this technique.

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