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The MPLEx Protocol for Multi-omic Analyses of Soil Samples
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JoVE Journal Chemistry
The MPLEx Protocol for Multi-omic Analyses of Soil Samples

The MPLEx Protocol for Multi-omic Analyses of Soil Samples

DOI:
10.3791/57343-v

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10:12 min

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May 30, 2018

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, , , , , , , , , , ,
1Biological Sciences Division,Pacific Northwest National Laboratory

Chapters

  • 00:04Title
  • 00:36Soil Cell Lysis and Extraction of Metabolites, Proteins, and Lipids
  • 07:55Results: Analysis of the MPLEx Protocol for Extraction of Soil Samples
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol is presented for simultaneously extracting metabolites, proteins, and lipids from a single soil sample, allowing reduced sample preparation times and enabling multi-omic mass spectrometry analyses of samples with limited quantities.

Tags

MPLEx ProtocolMulti-omic AnalysesSoil SamplesProteomicsMetabolomicsLipidomicsMicrobial CommunitiesEnvironmental PerturbationsSample PreparationChloroform-methanol ExtractionVortexingSonicationCentrifugationMetabolite LayerProtein InterlayerLipid Layer

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