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Chemistry
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土壤样品多涨价可能分析的 MPLEx 协议
JoVE Journal
Chemistry
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JoVE Journal
Chemistry
The MPLEx Protocol for Multi-omic Analyses of Soil Samples
Please note that all translations are automatically generated.
Click here for the English version.
土壤样品多涨价可能分析的 MPLEx 协议
DOI:
10.3791/57343-v
•
10:12 min
•
May 30, 2018
•
Carrie D. Nicora*
1
,
Kristin E. Burnum-Johnson*
1
,
Ernesto S. Nakayasu
,
Cameron P. Casey
,
Richard A. White III
,
Taniya Roy Chowdhury
,
Jennifer E. Kyle
,
Young-Mo Kim
,
Richard D. Smith
,
Thomas O. Metz
,
Janet K. Jansson
,
Erin S. Baker
1
Biological Sciences Division
,
Pacific Northwest National Laboratory
Chapters
00:04
Title
00:36
Soil Cell Lysis and Extraction of Metabolites, Proteins, and Lipids
07:55
Results: Analysis of the MPLEx Protocol for Extraction of Soil Samples
09:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了一种从单个土壤样品中同时提取代谢物、蛋白质和脂质的协议, 允许缩短样品准备时间, 并对数量有限的样品进行多涨价可能质谱分析。
Tags
MPLEx Protocol
Multi-omic Analyses
Soil Samples
Proteomics
Metabolomics
Lipidomics
Microbial Communities
Environmental Perturbations
Sample Preparation
Chloroform-methanol Extraction
Vortexing
Sonication
Centrifugation
Metabolite Layer
Protein Interlayer
Lipid Layer
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