Journal
/
Bioengineering
/
A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

DOI:
10.3791/57348-v

•

09:00 min

•

May 02, 2018

•

,
1Department of Physics Education,Kongju National University, 2Department of Physics,Harvard University, 3FAS Center for Systems Biology,Harvard University

Chapters

  • 00:04Title
  • 00:58Microfluidic Environment Preparation
  • 03:35Microfluidic Device Bacteria Loading
  • 04:53Microfluidic Device Worm Loading
  • 06:50Results: Representative Gene Expression and Egg Laying Kinetics
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

In this article, we demonstrate live imaging of individual worms employing a custom microfluidic device. In the device, multiple worms are individually confined to separate chambers, allowing multiplexed longitudinal surveillance of various biological processes.

Tags

Microfluidic PlatformCaenorhabditis ElegansLongitudinal ImagingEnvironmental ConditionsWorm Behavioral ResponsesGene ExpressionAdaptationMathematical ModelingHypothesis TestingSyringe TubeAdapter NeedleBuffer ExchangeS-mediumDevice OutletBuffer InletWorm Inlet

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

High-throughput Protein Expression Generator Using a Microfluidic Platform

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans‘ Locomotion

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Immobilization of Live Caenorhabditis elegans Individuals Using an Ultra-thin Polydimethylsiloxane Microfluidic Chip with Water Retention

Read Article