/
/
Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:56Creating a Dilution Library
- 05:03Adding Media-seed Mixture to Screening Plates
- 06:40Adding Small Molecules to Screening Plates
- 10:16Results: Representative Results from a Forward Chemical Genetics Screen on Arabidopsis
- 11:05Conclusion
A high throughput screen of synthetic small molecules was conducted on the model plant species, Arabidopsis thaliana. This protocol, developed for a liquid handling robot, increases the speed of forward chemical genetics screens, accelerating the discovery of novel small molecules affecting plant physiology.
