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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
JoVE Journal
Genetics
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JoVE Journal Genetics
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
DOI:

11:13 min

April 10, 2018

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Chapters

  • 00:04Title
  • 01:00Molecular Cloning
  • 02:23Kinetochore-microtubule Attachment Assay
  • 04:57Chromosome Alignment Challenge Assay
  • 08:52Results: Assessment of Human Gene Function During Meiosis in Mouse Oocytes
  • 10:12Conclusion

Summary

Automatic Translation

As the genetic variants associated with human disease begin to become uncovered, it is becoming increasingly important to develop systems with which to rapidly evaluate the biological significance of those identified variants. This protocol describes methods for evaluating human gene function during female meiosis I using mouse oocytes.

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