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Journal / Cancer Research / Desthiobiotin-streptavidina-affinità mediata…
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JoVE Journal Cancer Research
Desthiobiotin-streptavidina-affinità mediata purificazione delle proteine che interagiscono con RNA nelle cellule di mesotelioma
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Desthiobiotin-streptavidina-affinità mediata purificazione delle proteine che interagiscono con RNA nelle cellule di mesotelioma

Article DOI: 10.3791/57516-v • 11:11 min • April 25th, 2018
April 25th, 2018 •
1, 1
1Laboratory of Molecular Oncology, Division of Thoracic Surgery, University Hospital Zurich

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Desthiobiotin etichettatura di un sintetico oligo RNA 25 nucleotidi, che contiene un motivo di elemento ricco di adenina (ARE), permette di grippaggio specifico di proteina citosolica sono vincolanti.

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Ricerca sul cancro problema 134 RNA-proteina RNA-pulldown non-codificanti RNA 3' UTR mesotelioma umani-antigene R
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