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JoVE Journal Cancer Research
Modelado de Osteosarcoma usando las células de vástago de Pluripotent inducido derivados del paciente el síndrome de Li-Fraumeni
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Modelado de Osteosarcoma usando las células de vástago de Pluripotent inducido derivados del paciente el síndrome de Li-Fraumeni

Article DOI: 10.3791/57664-v • 08:52 min • June 13th, 2018
June 13th, 2018 •
1,2, 1, 1,3, 1, 4, 1, 1,2,5,6
1Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, 2Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, 3Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, 4Women's Health Institute, Cleveland Clinic Foundation, 5Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, 6Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

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Aquí, presentamos un protocolo para la generación de pluripotentes inducidas (iPSCs) de células de fibroblastos derivados pacientes de síndrome de Li-Fraumeni (LFS), diferenciación de iPSCs a través de las células madre mesenquimales (MSCs) y osteoblastos, modelado en vivo tumorigenesis con osteoblastos derivados de paciente LFS.

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Investigación de cáncer número 136 iPSCs síndrome de Li-Fraumeni reprogramación diferenciación las células madre mesenquimales osteoblastos osteosarcoma xenoinjerto
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