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Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

DOI:
10.3791/57684-v

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14:55 min

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September 01, 2018

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, , ,
1Department of Obstetrics and Gynecology, Center for Reproductive Health Sciences,Washington University School of Medicine

Chapters

  • 00:00Title
  • 00:56Human Endometrial Stromal Cell Isolation
  • 06:47Human Endometrial Stromal Cell siRNA Transfection
  • 10:06Human Endometrial Stromal Cell In Vitro Decidualization
  • 12:07Results: Decidualization of Human Endometrial Stromal Cells
  • 14:29Conclusion

Summary

Automatic Translation

Automatic Translation

This study presents a validated and optimized procedure for the isolation and culture of human endometrial stromal cells to conduct in vitro decidualization assay. Further, this study provides a detailed method to efficiently knockdown a specific gene using siRNAs in human endometrial stromal cells.

Tags

Human Endometrial Stromal CellsIn Vitro DecidualizationReproductive MedicineDecidualization ProtocolGene KnockdownProlactinInsulin-like Growth Factor Binding Protein OneHBSFHES Isolation MediaDNase-1Collagenase DigestionCell StrainerCell Culture

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