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A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
JoVE Journal
Medicine
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JoVE Journal Medicine
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
DOI:

07:14 min

July 13, 2018

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Chapters

  • 00:04Title
  • 00:42Establishment of Hypoxia/Reoxygenation Model
  • 01:57Preparation of HCMs for Flow Cytometry
  • 03:49Flow Cytometer Setup
  • 04:43Flow Cytometry Measurement and Data Analysis
  • 05:40Results: Validation of the Establishment of Hypoxia/Reoxygenation (H/R) Model by Flow Cytometry
  • 06:34Conclusion

Summary

Automatic Translation

Here, we present a protocol that uses JC-1 dye to assess the mitochondrial membrane potential of cells after being exposed to hypoxia/reoxygenation with or without a protective agent.

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