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Assessment of Kidney Function in Mouse Models of Glomerular Disease
JoVE Journal
Medicine
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JoVE Journal Medicine
Assessment of Kidney Function in Mouse Models of Glomerular Disease

Assessment of Kidney Function in Mouse Models of Glomerular Disease

DOI:
10.3791/57764-v

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09:16 min

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June 30, 2018

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,
1Institute of Biomedical and Clinical Sciences, Medical School,University of Exeter, 2School of Physiology, Pharmacology and Neurosciences,University of Bristol, 3Bristol Renal, School of Clinical Sciences,University of Bristol

Chapters

  • 00:04Title
  • 00:30Sample Collection and Preparation for Analysis
  • 03:27Glomeruli Isolation and Glomerular Water Permeability (LpA/Vi)
  • 05:36Periodic Acid Schiff (PAS) Stain
  • 06:55Results: Representative Kidney Function Assessment
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a full kidney work-up that should be carried out in mouse models of glomerular disease. The methods allow for detailed functional, structural, and mechanistic analysis of glomerular function, which can be applied to all mouse models of glomerular disease.

Tags

Kidney FunctionMouse ModelGlomerular DiseaseUrinary Albumin Creatinine RatioCortical GlomeruliJuxtamedullary GlomeruliHistologyImmunofluorochemical AnalysisProtein AnalysisRNA AnalysisGlomeruli Isolation

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