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Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
JoVE Journal
Genetics
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JoVE Journal Genetics
Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
DOI:

09:28 min

May 25, 2018

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Chapters

  • 00:05Title
  • 00:40Sample Fixation and Digestion
  • 03:50Hybridization
  • 05:03Washing and Preparing Cells for Imaging
  • 07:03Results: smFISH Detects Individual mRNA Isoforms of NDC80 in Single Cells
  • 08:40Conclusion

Summary

Automatic Translation

This single molecule fluorescence in situ hybridization protocol is optimized to quantify the number of RNA molecules in budding yeast during vegetative growth and meiosis.

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