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Journal
/
Developmental Biology
/
Preparation of
Drosophila
Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Preparation of
Drosophila
Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics
Preparation of
Drosophila
Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics
DOI:
10.3791/57847-v
•
07:21 min
•
June 06, 2018
•
Hongde Li
,
Jason M. Tennessen
1
Department of Biology
,
Indiana University
Chapters
00:05
Title
00:37
Larval Sample Collection
01:28
Sample Transfer to Bead Tubes
02:23
Sample Extraction
03:52
Chemical Derivatization
06:20
Results: Gas Chromatography-Mass Spectrometry Analyses Reveal Significant Differences Between Lactate Dehydrogenase Mutants and Wild-type Controls
07:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
This protocol describes how to prepare
Drosophila
larvae for GC-MS-based metabolomic analysis.
Tags
Drosophila
Larval Samples
Gas Chromatography-mass Spectrometry (GC-MS)
Metabolomics
L2-hydroxyglutarate
Sodium Chloride
Centrifugation
Liquid Nitrogen
Bead Tube
Methanol
Succinic-D4 Acid
Bead Mill Homogenizer
Article
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