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An In Vitro Approach to Photodynamic Therapy
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Medicine
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JoVE Journal Medicine
An In Vitro Approach to Photodynamic Therapy

An In Vitro Approach to Photodynamic Therapy

DOI:
10.3791/58190-v

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04:53 min

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August 17, 2018

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1Department of Dermatology,University of California, Davis, 2Dermatology Service,Sacramento VA Medical Center, 3Department of Dermatology,State University of New York, Downstate Medical Center

Chapters

  • 00:04Title
  • 00:37Blue Light Photodynamic Therapy (PDT)
  • 02:32Flow Cytometric Analysis
  • 03:39Results: Representative Efficacy of 5-ALA + PDT Treatment on Apoptosis
  • 04:23Conclusion

Summary

Automatic Translation

Automatic Translation

Photodynamic therapy (PDT) is a medical procedure that involves incubation of an exogenously applied photosensitizer (PS) followed by visible light photoactivation to induce apoptosis. We present an in vitro PDT protocol designed to simulate PDT that may be used to study differences in PS incubation and light treatment parameters.

Tags

Photodynamic TherapyPDTIn VitroPhotosensitizer5-ALABlue Light IrradiationCell CultureCell PlatingTemperature ControlFluenceCell DetachmentFlow Cytometry

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