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Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment
DOI:

08:59 min

August 03, 2018

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Chapters

  • 00:04Title
  • 00:57Low Density Neutrophil (LDN) Abdominal Cavity Lavage Isolation
  • 03:44Neutrophil Extracellular Trap (NET) Detection and Tumor Cell Adhesion Analysis
  • 06:37Results: Representative LDN: Tumor Cell Attachment Analyses
  • 08:16Conclusion

Summary

Automatic Translation

Here, we present a method in which human low-density neutrophils (LDN), recovered from postoperative peritoneal lavage fluid, produce massive neutrophil extracellular traps (NETs) and efficiently trap free tumor cells that subsequently grow.

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