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Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment
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Cancer Research
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JoVE Journal Cancer Research
Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment

Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment

DOI:
10.3791/58201-v

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08:59 min

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August 03, 2018

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, , , , , , ,
1Department of Gastrointestinal Surgery,Jichi Medical University

Chapters

  • 00:04Title
  • 00:57Low Density Neutrophil (LDN) Abdominal Cavity Lavage Isolation
  • 03:44Neutrophil Extracellular Trap (NET) Detection and Tumor Cell Adhesion Analysis
  • 06:37Results: Representative LDN: Tumor Cell Attachment Analyses
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a method in which human low-density neutrophils (LDN), recovered from postoperative peritoneal lavage fluid, produce massive neutrophil extracellular traps (NETs) and efficiently trap free tumor cells that subsequently grow.

Tags

Neutrophil Extracellular TrapsNETsLow Density NeutrophilsPeritoneal Lavage FluidTumor Cell GrowthTumor MetastasisNeutrophil SubpopulationsTumor ImmunologyDensity Gradient SeparationMagnetic Activated Cell SortingMACSCD66bGastrointestinal Malignancy

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