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Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
DOI:

10:07 min

October 24, 2018

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Chapters

  • 00:04Title
  • 00:41Step I: Corneal Induction in Suspension Culture
  • 03:07Step II: Corneal Differentiation in Adherent Culture
  • 04:33Step III: Cryo-banking Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
  • 06:08Step IV: Thawing the Cryopreserved Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
  • 07:25Results: Differentiation of Human Pluripotent Stem Cells to Clinically Compatible Corneal Limbal Epithelial Stem Cells
  • 09:31Conclusion

Summary

Automatic Translation

This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.

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