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Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics
DOI:

12:28 min

January 11, 2019

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Chapters

  • 00:04Title
  • 01:03Generation of DR3 Overexpression Cell Lines
  • 05:41Verification of the DR3 Overexpression Cell Line
  • 07:46Assessment of the Apoptotic Response of Cells to Antimitotic Agents
  • 09:51Results: Assessment of the Apoptotic Response to Anti-mitotic Therapeutics
  • 11:15Conclusion

Summary

Automatic Translation

Establishing a stable cell line overexpressing a gene of interest to study gene function can be done by stable transfection-picking single clones after transfecting them via retroviral infection. Here we show that HT29-DR3 cell lines generated in this way elucidate the mechanisms by which death receptor 3 (DR3) contributes to antimitotics-induced apoptosis.

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