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Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics
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Chapters
- 00:04Title
- 01:03Generation of DR3 Overexpression Cell Lines
- 05:41Verification of the DR3 Overexpression Cell Line
- 07:46Assessment of the Apoptotic Response of Cells to Antimitotic Agents
- 09:51Results: Assessment of the Apoptotic Response to Anti-mitotic Therapeutics
- 11:15Conclusion
Establishing a stable cell line overexpressing a gene of interest to study gene function can be done by stable transfection-picking single clones after transfecting them via retroviral infection. Here we show that HT29-DR3 cell lines generated in this way elucidate the mechanisms by which death receptor 3 (DR3) contributes to antimitotics-induced apoptosis.
