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Journal / Biochemistry / Rad51 tarafından aracılı DNA Strand Exchange…
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Biochemistry

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JoVE Journal Biochemistry
Rad51 tarafından aracılı DNA Strand Exchange tepki gerçek zamanlı gözlem
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Rad51 tarafından aracılı DNA Strand Exchange tepki gerçek zamanlı gözlem

Article DOI: 10.3791/59073-v • 06:24 min • February 13th, 2019
February 13th, 2019 •
1, 1, 1, 1
1Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology

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DNA'ın sistemleri tarafından Rad51 aracılı Satım tepki strand floresans rezonans enerji transferi tabanlı gerçek zamanlı gözlem için geliştirilmiştir. Burada sunulan iletişim kurallarını kullanarak, biz tepki ara ürün ve ayrıca reaksiyon enzimatik kinetik analiz ederken onların dönüşüm ürünlerde, oluşumu tespit edebiliyoruz.

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Biyokimya sayı: 144 Homolog rekombinasyon DNA strand Satım tepki Rad51 Swi5-Sfr1 gerçek zamanlı gözlem floresans rezonans enerji transferi (FRET)
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