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3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
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Bioengineering
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JoVE Journal Bioengineering
3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

DOI:
10.3791/59226-v

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05:57 min

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May 24, 2019

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, , , ,
1Department of Biomedical Engineering and Yale Systems Biology Institute,Yale University, 2Department of Biomedical Engineering,Johns Hopkins University, 3Center for Cell Dynamics and Department of Cell Biology,Johns Hopkins University

Chapters

  • 00:04Title
  • 00:34Mesofluidic Device Fabrication
  • 02:20Collagen Injection, 3D Imaging, and Quantification
  • 04:20Results: Representative Ligand Gradient Evaluation and Cellular Gradient Reponses
  • 05:23Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method to construct devices for 3D culture and experimentation with cells and multicellular organoids. This device allows analysis of cellular responses to soluble signals in 3D microenvironments with defined chemoattractant gradients. Organoids are better than single cells at detection of weak noisy inputs.

Tags

3D AnalysisMulti-cellular ResponseChemoattractant GradientsPDMS DeviceMesofluidic DeviceStereo LithographyVacuum DegassingPDMS CuringCell LoadingCollagen Cell SuspensionCell Culture3D Physiological Environment

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