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Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
DOI:

09:10 min

June 25, 2019

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Chapters

  • 00:04Title
  • 00:36Preparation of Cell (COS1) Aggregates Genetically-modified to Secrete a Candidate Molecule in 3-D collagen Hydrogels
  • 04:03Generation of Embryonic Explant for Culture
  • 05:54Preparation of 3-D Co-cultures in Collagen Hydrogels
  • 07:25Results: Analysis of Axonal Growth and Behavior
  • 08:39Conclusion

Summary

Automatic Translation

Here, we provide a method for analyzing the behavior of growing axons in 3D matrices, mimicking their natural development.

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