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Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
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Neuroscience
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JoVE Journal Neuroscience
Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development

Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development

DOI:
10.3791/59481-v

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09:10 min

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June 25, 2019

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,
1Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST),Parc Científic de Barcelona, 2Department of Cell Biology, Physiology, and Immunology,Universitat de Barcelona, 3Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), 4Institute of Neuroscience,University of Barcelona

Chapters

  • 00:04Title
  • 00:36Preparation of Cell (COS1) Aggregates Genetically-modified to Secrete a Candidate Molecule in 3-D collagen Hydrogels
  • 04:03Generation of Embryonic Explant for Culture
  • 05:54Preparation of 3-D Co-cultures in Collagen Hydrogels
  • 07:25Results: Analysis of Axonal Growth and Behavior
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we provide a method for analyzing the behavior of growing axons in 3D matrices, mimicking their natural development.

Tags

3D Collagen HydrogelsAxonal GrowthNervous System DevelopmentAxonal GuidanceNeuronal MigrationCOS-1 CellsDNA TransfectionLiposome-based TransfectionCollagen MixtureCell Pellet3D Cell Culture

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