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Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification
DOI:

09:17 min

July 12, 2019

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Chapters

  • 00:04Title
  • 00:47Adult Mouse Hind Limb Distal P3 Amputation
  • 02:01Digit Collection and Tissue Preparation
  • 02:47Immunostaining for Blastema Formation and Intramembranous Ossification
  • 04:30Sequential In Vivo Microcomputed Tomography (microCT) Imaging
  • 05:50Reconstructed Bone Image Analysis
  • 08:15Results: Representative Regenerative Bone Analyses
  • 08:47Conclusion

Summary

Automatic Translation

Here, we present a protocol of adult mouse terminal phalanx amputation to investigate mammalian blastema formation and intramembranous ossification, analyzed by fluorescent immunohistochemistry and sequential in-vivo microcomputed tomography.

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