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Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
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Chapters
- 00:04Title
- 00:46Human Umbilical Cord Blood Mononuclear Cell (MNC) Isolation
- 02:45CD34+ Cell Isolation from MNCs
- 04:55Flow Cytometric Analysis of Hematopoietic Stem and Progenitor Cell (HSPC) Populations
- 05:59CD34+ HSPC Myeloid Differentiation
- 08:06Results: Representative Characterization of Freshly-isolated and Differentiated HSPCs
- 09:47Conclusion
Here, we present a protocol for immunophenotypic characterization and cytokine induced differentiation of cord blood derived CD34+ hematopoietic stem and progenitor cells to the four myeloid lineages. The applications of this protocol include investigations on the effect of myeloid disease mutations or small molecules on myeloid differentiation of the CD34+ cells.
