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Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
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Cancer Research
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JoVE Journal Cancer Research
Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

DOI:
10.3791/59871-v

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08:42 min

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July 19, 2019

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, , ,
1State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine,Sun Yat-sen University Cancer Center

Chapters

  • 00:04Title
  • 01:18Establishment of PDX Model
  • 02:22Tissue Cryopreservation
  • 04:48Isolation of Primary Cells
  • 07:13Results: Analysis of Established Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

The current protocol describes methods to establish patient-derived xenograft (PDX) models and primary cancer cell lines from surgical gastric cancer samples. The methods provide a useful tool for drug development and cancer biology research.

Tags

Patient-derived Xenograft ModelsPrimary Cell LinesTumor Biology ResearchDrug DevelopmentCancer Cell LinesNSG MiceTumor MicroenvironmentTumor DissectionTumor Cryopreservation

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