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High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
DOI:

11:31 min

August 16, 2019

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Chapters

  • 00:04Title
  • 01:00Transfection of Viral Producer Cells
  • 03:34Primary CD8 T Cell Isolation and Activation
  • 07:10Transduction of Activated CD8 T Cells
  • 09:29Results: Analysis of Transduced CD8 CAR-T Cells
  • 10:42Conclusion

Summary

Automatic Translation

This article describes a protocol for the generation of antigen-specific CD8 T cells, and their expansion in vitro, with the aim of yielding high numbers of functional T cells for use in vitro and in vivo.

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