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Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets
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Chapters
- 00:04Title
- 00:44Target Microsphere Preparation
- 02:20Antibody-Dependent Cellular Phagocytosis (ADCP) Assay Plate Preparation
- 02:53Breast Milk Cell Isolation
- 03:48ADCP Assay
- 05:11Flow Cytometric Analysis
- 06:21Results: Representative Flow Cytometric and ADCP Analyses of HIV Targets
- 07:42Conclusion
Breast milk transmits human immunodeficiency virus (HIV), though only ~15% of infants breastfed by HIV-infected mothers become infected. Breastfed infants ingest ~105−108 maternal leukocytes daily, though these cells are understudied. Here we describe the isolation of breast milk leukocytes and an analysis of their phagocytic capacity.
